Hi Azita,
the problem in your circuit seems to be the value of R3. you can come up with a 1uA by replacing R3 with 12.5k value.
you also need to lower the value of your Rload(R6) to atleast 2k.
You can use the following equation in getting the desired current(I) value base on the circuit you provided:
I = Vref*R2/(R1*R3)
where R2 = R4 and R1=R5
Vref = input voltage to the Howland current pump
and I = desired current output
please let us know if this helps.
Regards,
Erbe